TRIM16 monoclonal antibody (M01), clone 5F4
产品名称： TRIM16 monoclonal antibody (M01), clone 5F4
英文名称： TRIM16 monoclonal antibody (M01), clone 5F4
产品编号： H00010626-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant TRIM16.
- Immunogen:
- TRIM16 (NP_006461, 165 a.a. ~ 273 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- LDAARRDKEAELQCTQLDLERKLKLNENAISRLQANQKSVLVSVSEVKAVAEMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMA
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of TRIM16 expression in transfected 293T cell line by TRIM16 monoclonal antibody (M01), clone 5F4.
Lane 1: TRIM16 transfected lysate(64 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunoprecipitation
- Immunoprecipitation of TRIM16 transfected lysate using anti-TRIM16 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TRIM16 MaxPab rabbit polyclonal antibody.
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged TRIM16 is approximately 0.03ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of TRIM16 over-expressed 293 cell line, cotransfected with TRIM16 Validated Chimera RNAi ( Cat # H00010626-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM16 monoclonal antibody (M01), clone 5F4 (Cat # H00010626-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 10626
- GeneBank Accession#:
- NM_006470
- Protein Accession#:
- NP_006461
- Gene Name:
- TRIM16
- Gene Alias:
- EBBP
- Gene Description:
- tripartite motif-containing 16
- Omim ID:
- 609505
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene was identified as an estrogen and anti-estrogen regulated gene in epithelial cells stably expressing estrogen receptor. The protein encoded by this gene contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. The proteins of this family have been reported to be involved in a variety of biological processes including cell growth, differentiation and pathogenesis. Expression of this gene was detected in most tissues. Its function, however, has not yet been determined. [provided by RefSeq
- Other Designations:
- estrogen-responsive B box protein