HSPA4 抗体
Applications
| Tested Applications: |
ELISA, WB, IHC, IF, IP |
| Cited applications |
ELISA |
| Specificity Reaction: |
Human,Mouse,Rat, Monkey; Other species are not tested.Please decide the specificity by homology |
| Cited species |
Human |
| Positive WB detected in |
L02 cells, A375 cells, HEK-293 cells, K-562 cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, mouse ovary tissue, mouse testis tissue, NIH/3T3 cells, rat brain tissue |
| Positive IP detected in |
Mouse brain tissue |
| Positive IHC detected in |
Human colon cancer tissue, human breast cancer tissue, human pancreas cancer tissue |
| Positive IF detected in |
HepG2 cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:10-1:100 |
HSPA4 抗体Application key: WB=Western Blotting ,IHC=Immunohistochemistry, IF=Immunofluorescence
Images
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L02 cells were subjected to SDS PAGE followed by western blot with 21206-1-AP(HSPA4 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using 21206-1-AP(HSPA4 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using 21206-1-AP(HSPA4 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using HSPA4 antibody 21206-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-HSPA4 (IP:21206-1-AP, 4ug; Detection:21206-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
Product Information
| Source: |
Rabbit |
Purify: |
Antigen affinity purification |
| IsoType: |
IgG |
Storage: |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. -20oC, Avoid freeze / thaw cycles. |
Immunogen Information HSPA4 抗体
| Immunogen: |
HSPA4 fusion protein ag15581 |
Full Name: |
Heat shock 70kDa protein 4 |
Caculated Molecular
Weight: |
840aa,94 kDa |
Observed Molecular
Weight: |
110-120kd |
| GenbankNo |
BC110861 |
GeneID: |
3308 |
| Symbol |
HSPA4 |
Synonyms |
APG 2, APG2, Heat shock 70 kDa protein 4, heat shock 70kDa protein 4, HS24/P52, HSP70.4, hsp70RY, HSPA4, RY |
Background
|
HSPA4 (also known as Apg-2, HSP70RY) is a 110 kDa cytosolic protein, a member of the heat-shock protein 110 (Hsp 110) subfamily of Hsp70 proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA4 is ubiquitously expressed and its expression is not heat inducible. Overexpression of HSPA4 has been reported in some leukemia and solid tumors. This antibody well recognized the endogenous HSPA4 protein in mouse brain/ testis tissues. ( 21487003) HSPA4 抗体
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