H3K4me2 polyclonal antibody
产品名称： H3K4me2 polyclonal antibody
英文名称： H3K4me2 polyclonal antibody
产品编号： PAB14111
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of H3K4me2.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the dimethylated lysine 4 (H3K4me2).
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Storage Buffer:
- In PBS (0.05% sodium azide, 0.05% proclin 300)
- Storage Instruction:
- Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:100-1:500)
Dot Blot (1:20000)
Western Blot (1:1000)
ChIP (5 ug/ChIP)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Dynamic regulation of histone lysine methylation by demethylases.
Shi Y, Whetstine JR.Mol Cell. 2007 Jan 12;25(1):1-14.
- 2.
- Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.
Zheng C, Hayes JJ.J Biol Chem. 2003 Jun 27;278(26):24217-24. Epub 2003 Apr 15.
- 3.
- Translating the histone code.
Jenuwein T, Allis CD.Science. 2001 Aug 10;293(5532):1074-80.
- Applications
- Western Blot (Cell lysate)
- Nuclear extracts of HeLa cells (15 ug) were analysed by Western blot using H3K4me2 polyclonal antibody (Cat # PAB14111) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
- ELISA
- ELISA was performed using a serial dilution of H3K4me2 polyclonal antibody (Cat # PAB14111), crude serum and flow through in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 18,300.
- Dot Blot
- A Dot Blot analysis was performed to test the cross reactivity of H3K4me2 polyclonal antibody (Cat # PAB14111) with peptides containing other modifications and unmodified sequences of histone H3.
One hundred to 0.2 pmol of the respective peptides were spotted on a membrane.
The antibody was used at a dilution of 1 : 20,000.
Figure shows a high specificity of the antibody for the modification of interest.