MAP2K7 (Human) Recombinant Protein
产品名称： MAP2K7 (Human) Recombinant Protein
英文名称： MAP2K7 (Human) Recombinant Protein
产品编号： P5587
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- 邮编 : 11493
- 所在区域 : 台湾
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- Specification
- Product Description:
- Human MAP2K7 (NP_660186.1, 1 a.a. - 419 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
- Theoretical MW (kDa):
- 75
- Form:
- Liquid
- Preparation Method:
- Baculovirus infected insect cell (Sf21) expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 90 % by SDS-PAGE/CBB staining
- Activity:
- The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG). Substrate: JNK1 [inactive mutant]. ATP: 100 uM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Loading 1 ug protein in SDS-PAGE
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 5609
- Protein Accession#:
- NP_660186.1
- Gene Name:
- MAP2K7
- Gene Alias:
- Jnkk2,MAPKK7,MKK7,PRKMK7
- Gene Description:
- mitogen-activated protein kinase kinase 7
- Omim ID:
- 603014
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined. [provided by RefSeq
- Other Designations:
- JNK kinase 2,JNK-activating kinase 2,MAP kinase kinase 7,OTTHUMP00000174397,c-Jun N-terminal kinase kinase 2,dual specificity mitogen-activated protein kinase kinase 7