P70S6K抗体Applications
| In-house applications |
ELISA, WB, IF, IP, FC, IHC Application not tested? Please see our Guarantee. |
| Published applications |
IHC, WB |
Publications |
4 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Human, mouse |
|
Images
Prev12Next
-Antibody-14485-1-AP-p48029wb.jpg "HeLa cells were subjected to SDS PAGE followed by western blot with 14485-1-AP(p70(S6K) antibody) at dilution of 1:1200") HeLa cells were subjected to SDS PAGE followed by western blot with 14485-1-AP(p70(S6K) antibody) at dilution of 1:1200-Antibody-14485-1-AP-IP-35809.jpg "IP Result of anti-p70(S6K) (IP:14485-1-AP, 3ug; Detection:14485-1-AP 1:1000) with HeLa cells lysate 3000ug.") IP Result of anti-p70(S6K) (IP:14485-1-AP, 3ug; Detection:14485-1-AP 1:1000) with HeLa cells lysate 3000ug.-Antibody-14485-1-AP-IHC-73219.jpg "Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14485-1-AP( p70(S6K) Antibody) at dilution of 1:200 (under 10x lens).") Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14485-1-AP( p70(S6K) Antibody) at dilution of 1:200 (under 10x lens).-Antibody-14485-1-AP-IHC-73220.jpg "Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14485-1-AP( p70(S6K) Antibody) at dilution of 1:200 (under 40x lens).") Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14485-1-AP( p70(S6K) Antibody) at dilution of 1:200 (under 40x lens).-Antibody-14485-1-AP-IF-17645.jpg "Immunofluorescent analysis of HepG2 cells using 14485-1-AP( p70(S6K) Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG") Immunofluorescent analysis of HepG2 cells using 14485-1-AP( p70(S6K) Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG-Antibody-14485-1-AP-FC-38056.jpg "1X10^6 HepG2 cells were stained with 0.2ug p70(S6K) antibody (14485-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.") 1X10^6 HepG2 cells were stained with 0.2ug p70(S6K) antibody (14485-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
|
| Positive WB detected in |
HeLa cells, HepG2 cells, Jurkat cells, rat spleen tissue |
| Positive IP detected in |
HeLa cells |
| Positive IHC detected in |
Human breast cancer tissue |
| Positive IF detected in |
HepG2 cells |
| Positive FC detected in |
HepG2 cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:100-1:400
IF : 1:10-1:100 |
P70S6K抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information P70S6K抗体
| Immunogen |
P70S6K fusion protein ag5883 |
Full name |
Ribosomal protein S6 kinase, 70kDa, polypeptide 1 |
Calculated molecular
weight |
59 kDa |
Observed molecular
weight |
70 kDa,90 kDa |
GenBank accession
number |
BC053365 |
Gene ID (NCBI) |
6198 |
| Gene symbol |
RPS6KB1 |
Synonyms |
P70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K) alpha, p70S6K, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A |
Background
|
RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway and it plays a key role in cell growth and proliferation by regulating insulin sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy.(PMID:22684641). It has 2 isoforms produced by alternative initiation. P70S6K抗体
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