ARAF polyclonal antibody
产品名称： ARAF polyclonal antibody
英文名称： ARAF polyclonal antibody
产品编号： PAB3478
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of ARAF.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to internal region of human ARAF.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Selective activation of MEK1 but not MEK2 by A-Raf from epidermal growth factor-stimulated Hela cells.
Wu X, Noh SJ, Zhou G, Dixon JE, Guan KL.J Biol Chem. 1996 Feb 9;271(6):3265-71.
- 2.
- The complete sequence and promoter activity of the human A-raf-1 gene (ARAF1).
Lee JE, Beck TW, Brennscheidt U, DeGennaro LJ, Rapp UR.Genomics. 1994 Mar 1;20(1):43-55.
- 3.
- Localization of the pKs gene, a raf related sequence on human chromosomes X and 7.
Popescu NC, Mark GE.Oncogene. 1989 Apr;4(4):517-9.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of MCF-7 cell lysate (35 ug/lane) with ARAF polyclonal antibody (Cat # PAB3478).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARAF polyclonal antibody (Cat # PAB3478) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 369
- Protein Accession#:
- P10398
- Gene Name:
- ARAF
- Gene Alias:
- A-RAF,ARAF1,PKS2,RAFA1
- Gene Description:
- v-raf murine sarcoma 3611 viral oncogene homolog
- Omim ID:
- 311010
- Gene Ontology:
- Hyperlink
- Other Designations:
- OTTHUMP00000023212,Oncogene ARAF1,Ras-binding protein DA-Raf,v-raf murine sarcoma 3611 viral oncogene homolog 1
- Interactome
- Gene Pathway
- Acute myeloid leukemia
- Bladder cancer
- Chronic myeloid leukemia
- Colorectal cancer
- Endometrial cancer
- ErbB signaling pathway
- Glioma
- Insulin signaling pathway
- Long-term depression
- Long-term potentiation
- Melanoma
- Natural killer cell mediated cytotoxicity
- Non-small cell lung cancer
- Pancreatic cancer
- Pathways in cancer
- Prostate cancer
- Regulation of actin cytoskeleton
- Renal cell carcinoma
- Vascular smooth muscle contraction